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px1 pcr plate sealer  (Bio-Rad)


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    Structured Review

    Bio-Rad px1 pcr plate sealer
    Px1 Pcr Plate Sealer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 783 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px1+plate+sealer/PX1+PCR+Plate+Sealer/pm42087187-205-16-20
    Average 96 stars, based on 783 article reviews
    px1 pcr plate sealer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Droplet digital PCR for growth kinetics of recombinant baculovirus expressing VP2 protein of porcine parvovirus
    Article Snippet: .. Plates were sealed with pierceable foil using the PX1 plate sealer (BioRad) at 180 oC for 3 s. C1000 touch thermal cycler (Bio-Rad) was used for the PCR amplification. ..

    Article Title: Tauroursodeoxycholic Acid Protects Retinal Ganglion Cells and Reduces Inflammation in Mice Following Optic Nerve Crush.
    Article Snippet: 6-fluorescein amidite (FAM) hydrolysis probe sets for ddPCR were ordered from Integrated DNA Technologies (Coralville, IA, USA) or Biorad (Hercules, CA, USA) or for each target and normalized to a hexachlorofluorescein (HEX) HPRT hydrolysis probe set (Integrated DNA Technologies). ddPCR was performed using 5 ng of cDNA and FAM hydrolysis probe sets for IL-1β (Mm.PT.58.41616450; Integrated DNA Technologies, IA, USA), IL6 (dMmuCPE5095532; Biorad, CA, USA), TNF-α (Mm.PT.58.12575861; Integrated DNA Technologies) and IL-10 (dMmuCPE5094026; Bio-Rad, Hercules, CA, USA), and a HEX hydrolysis probe set for HPRT (Mm.PT.39a22214828; Integrated DNA Technologies, IA, USA) in Biorad ddPCR Supermix for Probes (no deoxyuridine triphosphate [dUTP]). .. The droplets were prepared using an Automated Droplet Generator model AutoDG (Biorad, CA, USA), plates were sealed with a PX1 plate sealer, PCR was performed using a C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA) with deep well block, and read using a QX200 droplet reader (Bio-Rad, Hercules, CA, USA). .. Data were analyzed using Bio-Rad QuantaSoft software v1.7.4.0917 and Microsoft (Redmond, WA, USA) Excel 2016.

    Article Title: Chimerism analysis by ABO blood group genotyping with digital droplet PCR.
    Article Snippet: Objective Chimerism analysis is an important post-transplant assessment for allogeneic hematopoietic stem cell transplant (HCT) recipients.. Although various chimerism analysis techniques are already established, they are limited in terms of sensitivity, versatility, and turnaround time.. Our objective was to develop a digital droplet polymerase chain reaction (ddPCR) assay for chimerism analysis using ABO gene polymorphisms as markers.

    Article Title: Tauroursodeoxycholic Acid Protects Retinal Ganglion Cells and Reduces Inflammation in Mice Following Optic Nerve Crush
    Article Snippet: 6-fluorescein amidite (FAM) hydrolysis probe sets for ddPCR were ordered from Integrated DNA Technologies (Coralville, IA, USA) or Biorad (Hercules, CA, USA) or for each target and normalized to a hexachlorofluorescein (HEX) HPRT hydrolysis probe set (Integrated DNA Technologies). ddPCR was performed using 5 ng of cDNA and FAM hydrolysis probe sets for IL-1β (Mm.PT.58.41616450; Integrated DNA Technologies, IA, USA), IL-6 (dMmuCPE5095532; Biorad, CA, USA), TNF-α (Mm.PT.58.12575861; Integrated DNA Technologies) and IL-10 (dMmuCPE5094026; Bio-Rad, Hercules, CA, USA), and a HEX hydrolysis probe set for HPRT (Mm.PT.39a22214828; Integrated DNA Technologies, IA, USA) in Biorad ddPCR Supermix for Probes (no deoxyuridine triphosphate [dUTP]). .. The droplets were prepared using an Automated Droplet Generator model AutoDG (Biorad, CA, USA), plates were sealed with a PX1 plate sealer, PCR was performed using a C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA) with deep well block, and read using a QX200 droplet reader (Bio-Rad, Hercules, CA, USA). .. Data were analyzed using Bio-Rad QuantaSoft software v1.7.4.0917 and Microsoft (Redmond, WA, USA) Excel 2016.

    Article Title: Development of a Duplex Droplet Digital Polymerase Chain Reaction Assay for Simultaneous Quantitative Detection of Two Erwinia Species
    Article Snippet: For droplet generation, 20 μl reaction mixture was transferred into an eight-channel DG8 cartridge (Bio-Rad), and 70 μl Droplet Generation Oil for Probes (Bio-Rad) was added to each oil well. .. The generated emulsion (40 μl) was transferred into a 96-well PCR plate (Bio-Rad) and sealed with a pierceable cover using a PX1 plate sealer (Bio-Rad). .. End-point PCR was performed using a C1000 thermal cycler (BioRad) with the following conditions: an initial denaturation of 98°C for 10 min, followed by 40 cycles of a two-step thermal profile consisting of 94°C for 30 sec and 52.8°C to 64.8°C for 60 sec at a ramp rate of 2.0oC/sec.

    Amplification:

    Article Title: Droplet digital PCR for growth kinetics of recombinant baculovirus expressing VP2 protein of porcine parvovirus
    Article Snippet: .. Plates were sealed with pierceable foil using the PX1 plate sealer (BioRad) at 180 oC for 3 s. C1000 touch thermal cycler (Bio-Rad) was used for the PCR amplification. ..

    Article Title: Chimerism analysis by ABO blood group genotyping with digital droplet PCR.
    Article Snippet: Objective Chimerism analysis is an important post-transplant assessment for allogeneic hematopoietic stem cell transplant (HCT) recipients.. Although various chimerism analysis techniques are already established, they are limited in terms of sensitivity, versatility, and turnaround time.. Our objective was to develop a digital droplet polymerase chain reaction (ddPCR) assay for chimerism analysis using ABO gene polymorphisms as markers.

    Blocking Assay:

    Article Title: Tauroursodeoxycholic Acid Protects Retinal Ganglion Cells and Reduces Inflammation in Mice Following Optic Nerve Crush.
    Article Snippet: 6-fluorescein amidite (FAM) hydrolysis probe sets for ddPCR were ordered from Integrated DNA Technologies (Coralville, IA, USA) or Biorad (Hercules, CA, USA) or for each target and normalized to a hexachlorofluorescein (HEX) HPRT hydrolysis probe set (Integrated DNA Technologies). ddPCR was performed using 5 ng of cDNA and FAM hydrolysis probe sets for IL-1β (Mm.PT.58.41616450; Integrated DNA Technologies, IA, USA), IL6 (dMmuCPE5095532; Biorad, CA, USA), TNF-α (Mm.PT.58.12575861; Integrated DNA Technologies) and IL-10 (dMmuCPE5094026; Bio-Rad, Hercules, CA, USA), and a HEX hydrolysis probe set for HPRT (Mm.PT.39a22214828; Integrated DNA Technologies, IA, USA) in Biorad ddPCR Supermix for Probes (no deoxyuridine triphosphate [dUTP]). .. The droplets were prepared using an Automated Droplet Generator model AutoDG (Biorad, CA, USA), plates were sealed with a PX1 plate sealer, PCR was performed using a C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA) with deep well block, and read using a QX200 droplet reader (Bio-Rad, Hercules, CA, USA). .. Data were analyzed using Bio-Rad QuantaSoft software v1.7.4.0917 and Microsoft (Redmond, WA, USA) Excel 2016.

    Article Title: Tauroursodeoxycholic Acid Protects Retinal Ganglion Cells and Reduces Inflammation in Mice Following Optic Nerve Crush
    Article Snippet: 6-fluorescein amidite (FAM) hydrolysis probe sets for ddPCR were ordered from Integrated DNA Technologies (Coralville, IA, USA) or Biorad (Hercules, CA, USA) or for each target and normalized to a hexachlorofluorescein (HEX) HPRT hydrolysis probe set (Integrated DNA Technologies). ddPCR was performed using 5 ng of cDNA and FAM hydrolysis probe sets for IL-1β (Mm.PT.58.41616450; Integrated DNA Technologies, IA, USA), IL-6 (dMmuCPE5095532; Biorad, CA, USA), TNF-α (Mm.PT.58.12575861; Integrated DNA Technologies) and IL-10 (dMmuCPE5094026; Bio-Rad, Hercules, CA, USA), and a HEX hydrolysis probe set for HPRT (Mm.PT.39a22214828; Integrated DNA Technologies, IA, USA) in Biorad ddPCR Supermix for Probes (no deoxyuridine triphosphate [dUTP]). .. The droplets were prepared using an Automated Droplet Generator model AutoDG (Biorad, CA, USA), plates were sealed with a PX1 plate sealer, PCR was performed using a C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA) with deep well block, and read using a QX200 droplet reader (Bio-Rad, Hercules, CA, USA). .. Data were analyzed using Bio-Rad QuantaSoft software v1.7.4.0917 and Microsoft (Redmond, WA, USA) Excel 2016.

    Generated:

    Article Title: Development of a Duplex Droplet Digital Polymerase Chain Reaction Assay for Simultaneous Quantitative Detection of Two Erwinia Species
    Article Snippet: For droplet generation, 20 μl reaction mixture was transferred into an eight-channel DG8 cartridge (Bio-Rad), and 70 μl Droplet Generation Oil for Probes (Bio-Rad) was added to each oil well. .. The generated emulsion (40 μl) was transferred into a 96-well PCR plate (Bio-Rad) and sealed with a pierceable cover using a PX1 plate sealer (Bio-Rad). .. End-point PCR was performed using a C1000 thermal cycler (BioRad) with the following conditions: an initial denaturation of 98°C for 10 min, followed by 40 cycles of a two-step thermal profile consisting of 94°C for 30 sec and 52.8°C to 64.8°C for 60 sec at a ramp rate of 2.0oC/sec.

    Emulsion:

    Article Title: Development of a Duplex Droplet Digital Polymerase Chain Reaction Assay for Simultaneous Quantitative Detection of Two Erwinia Species
    Article Snippet: For droplet generation, 20 μl reaction mixture was transferred into an eight-channel DG8 cartridge (Bio-Rad), and 70 μl Droplet Generation Oil for Probes (Bio-Rad) was added to each oil well. .. The generated emulsion (40 μl) was transferred into a 96-well PCR plate (Bio-Rad) and sealed with a pierceable cover using a PX1 plate sealer (Bio-Rad). .. End-point PCR was performed using a C1000 thermal cycler (BioRad) with the following conditions: an initial denaturation of 98°C for 10 min, followed by 40 cycles of a two-step thermal profile consisting of 94°C for 30 sec and 52.8°C to 64.8°C for 60 sec at a ramp rate of 2.0oC/sec.



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